An open-source Python library for the analysis of luminescence lifetime and hyperspectral images using the phasor approach.
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Nov 7, 2026 - Python
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An open-source Python library for the analysis of luminescence lifetime and hyperspectral images using the phasor approach.
python image-analysis luminescence phasor fluorescence-microscopy-imaging hyperspectral-imaging flim fluorescence-lifetime
Fluorescence Lifetime Ultimate Explorer (FLUTE) is a GUI for exploring and analyzing Fluorescence Lifetime Microscopy (FLIM) data
🪻 A Huge Collection of Compositor Nodes for Blender
blender photography effects image-processing lightroom photo-editing nodes compositor color-space color-management tone-mapping color-grading aces flim view-transform
File format agnostic low level, high performance API to read and process time-tagged-time resolved (TTTR) data for single-molecule and image spectroscopy)
python single-molecule fluorescence imaging fluorescence-microscopy-imaging ptu flim becker fluorescence-correlation picoquant
Alternative FLIM fitting/phasor analysis for FLIM files
python gui analysis sdt gpu-computing lifetime ptu flim lif lifetime-distribution exponential-reconvolution ifli flimkit flimkit-plugin
Read and write PicoQuant PTU and related files (PHU, PCK, PCO, PFS, PUS, PQRES, PQDAT, PQUNI, SPQR, and BIN).
python format-reader life-sciences-image fluorescence-microscopy-imaging ptu flim tcspc picoquant tttr photon-counting fluorescence-lifetime-spectroscopy
FLIM-focused framework for multimodal single-cell imaging: extract features from FLIM and QPI data, then analyze any tabular dataset interactively.
python tabular-data scientific-visualization feature-extraction data-analysis microscopy umap cell-analysis flim quantitative-phase-imaging single-cell-imaging single-cell-analysis bioimage-analysis streamlit multimodal-imaging multimodal-analysis phasor-analysis fluorescence-lifetime-imaging
Read Leica image files (LIF, LOF, XLIF, XLEF, XLCF, XLLF, and LIFEXT).
python image format-reader life-sciences-image fluorescence-microscopy-imaging flim leica fluorescence-lifetime-spectroscopy leica-lif leica-xlef
Read Becker & Hickl SDT files.
python format-reader life-sciences-image fluorescence-microscopy-imaging flim photon-counting fluorescence-lifetime-spectroscopy
FLIMPA: An open-sourced software for phasor plot analyis of TCSPC-FLIM data
Deep learning for fluorescence lifetime predictions
machine-learning deep-learning regression yolo fluorescence microscopy lifetime flim fluorescence-lifetime yolov5
ImageJ plugin reading PicoQuant ptu/pt3 FLIM image files
converter stack imagej imagej-plugins lifetime fiji-plugin flim utrecht-university pt3 picoquant fluorescence-lifetime
A MATLAB data analysis toolkit for Fluorescence Lifetime Imaging Microscopy (FLIM) and an Alba v5 time-resolved confocal microscope operations manual
FLIM Digital Twin
CLI application for reading and creating FLIM libraries. The standard library will be updated all the time. The original library can serve as a repository of data about files by name, this provides quick access to the files you need.
filemanager file-manager cli-application library-management library-management-system flim flim-libraries
Automatic segmentation of intravital fluorescence microscopy images by K-means clustering of FLIM phasors
image-segmentation k-means phasor fluorescence-microscopy-imaging matlab-gui flim lifetime-spectroscopy
[Experimental] TCSPC data processing library
Python reconvolution fitting for freely available public TCSPC decay data, with IRF-aware lifetime estimation and diagnostics.
python scientific-computing fit convolution fluorescence imaging deconvolution flim tcspc reconvolution fluorescence-lifetime single-photon
Maximum likelihood fits for low photon count data - For active develeopment visit gitlab.peulen.xyz
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